The malic dehydrogenase of animal tissues

Diphosphopyridine nucleotide isocitric dehydrogenase from animal tissues.

The Inhibition of Fumarase and Malic Dehydrogenase

The competitive inhibition of malic dehydrogenase by /3-fluorooxaloacetate has been reported by Kun et al. (1). In addition to its inhibitory effect, /3-fluoro-oxaloacetate also oxidized reduced diphosphopyridine nucleotide enzymatically, but at a rate only 0.02% that of oxaloacetate. It was also shown (2) that fi-fluorooxaloacetate was a competitive inhibitor of glutamic-aspartic transaminase,...

The assay of animal tissues for respiratory enzymes; the malic dehydrogenas system.

The “homogenate technique” is at present widely used for the study of enzymatic reactions in various types of biological material. In the original technique (1)’ an attempt n-as made to eliminat,e cell structure and to disperse intracellular constitucnt,s so widely that endogenous respiration was eliminated by dilution, while particular phases of metabolism could be restored by the addition of ...

Contamination of malic dehydrogenase with apotransaminase.

TRANSAMINASES are among the enzyme requiring pyridoxal-5 phosphate as a co-factor (1). Tn the course of studies on the presence of the apoenzyme of glutamic oxaloacetic transaminase (GOT) in the serum, it was found that the malic dehydrogenase preparations, which are used in the Karmen (2) transaminase assay system, had variable amounts of apotransaminase. The studies reported below present the...

Isolation and properties of malic dehydrogenase from ox-heart mitochondria.

Ghiretti, F. & Libonati, M. (1957). Acta Embryol. Morphol. Exper. 1, 48. Ghiretti-Magaldi, A., Giuditta, A. & Ghiretti, F. (1957). Pubbl. Staz. zool. Napoli (in the Press.) Keilin, D. (1925). Proc. Roy. Soc. B, 98, 312. Keilin, D. & Hartree, E. F. (1937). Proc. Roy. Soc. B, 122, 298. Keilin. D. & Hartree, E. F. (1939). Proc. Roy. Soc. B, 127, 167. Loewus, F. A., Westheimer, F. H. & Vennesland, ...